Monday, March 09, 2009

We Now Have A Bigger DNA Dataset For The Mummichog Project

Kris acquired the new, improved version of Geneious, the software package we've been using to align and interpret DNA data. With this software our usable stretch of cyt-b DNA has increased from 272 to 719 bases because it can read what had seemed to be garbled sequences to the old software. So that's a big step forward, given that the whole gene is ~1160 bases long (and I'd thought that our PCR/sequencing work was better than 272 bases, anyway). We might also be able to recover a sequence from a Boston Harbor fish, one of the first ones I captured back in 1995 in front of UMass/Boston. That makes me about as happy as you can get about DNA data....

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