Monday, February 16, 2009

We Started The Stippled Studfish DNA Purification Today

I met with Kris, Travis and Andrew today. We had to make sure that we had all of the necessary reagents for the Qiagen kit purification process (we did, after begging some PB reagent from the Podila lab), and Kris explained his slight tricks with the generally good Qiagen documentation of purification. We did a test run on 6 of our samples in the Bishop lab, which does indeed have an Eppendorf centrifuge with variable speed control (a strange luxury). We weren't able to do a concentration test since no one was in the Podila lab late in the afternoon, cutting us off from the Nanodrop spec. If we get decent results, it's probably about 1.5 hours worth of work to purify our remaining samples. James is going to Tuscaloosa on Friday, so hopefully we can send these samples down with him and he can hand them off to people he knows in Phil Harris' lab.

I heard from Stan Sung yesterday. He and his pals are planning another Alabama trip in April. I might do a day trip with them down to Tallapoosa County to look at stippled studfish. If so, I hope to collect some Tallapoosa shiners for my aquaria. We'll see.

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