A Western Blot From Today

I'm not even sure which fish are on this blot, but it's one of three that Andrew, Alan and Selina worked on over the past two days. The bands of interest are the first major row down from the top, which is subunit 1 of the NMDA receptors in the various fish brains. The first lane on the left is mostly empty because what they used as a positive control doesn't really work; lane 10 on the right is the size ladder, itself a positive control of sorts. Andrew will scan the three blots on Monday and get our densitometry numbers. The blots contain a mix of silverstripe shiners from Tuesday, telescope and scarlet shiners from Estill Fork last November, and three of our most recent telescope and scarlet individuals from the last blot for comparison. The basic hypothesis is that fish from last fall should show relatively low NMDA since it's after breeding season, and we expect silverstripe shiners to be very similar to telescopes since they're closely related. But, we'll see...