Toting Up The Bifax Project To Date
Travis has caught up with the PCR and electrophoresis of our available DNA extractions on the stippled studfish project. The fish and locations we're working with are as follows:
4 fish @Cornhouse Creek, location #1
4 fish @Cornhouse Creek, location #2
4 fish @Elkahatchee Creek
4 fish @Hillabee Creek, location #1
4 fish @Hillabee Creek, location #2
2 fish @Broken Arrow Creek
2 fish @Emuckfaw Creek
4 fish @Sofkahatchee Creek
1 fish @Channahatchee Creek
Total: 29 stippled studfish, plus:
1 F. olivaceus from Broken Arrow Creek
3 F. stellifer from the Conasauga River in Tennessee
3 F. catenatus from Estill Fork in Alabama
1 Lythrurus bellus from Cornhouse Creek
for a grand total of 37 fish that we can hopefully get good sequences for. The L. bellus, a shiner, qualifies as a serious outgroup for phylogenetic comparison. We may, of course, find other stippled studfish on our next trip through Cleburne County, AL, but I'm dubious. I also heard from Rex Strange at Southern Indiana that he has cyt-b sequences he'll give me from F. julisia and some of the other studfishes too, so that would flesh out my analysis once we get our sequences.
And I still have to write the telescope shiner project into a journal article, preferably soon!
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