Wednesday, July 23, 2008

DNA Extractions In Gear

Travis has been working on extracting PCR-amplified stippled studfish DNA from gel cut-outs. Fourteen of 37 samples have been done now. This basically prepares the DNA for sequencing, which we can hopefully send out in the next several weeks at the most. We still have to test the DNA concentration in the samples but I have faith it will be good (and we'll know after we do it!).

My slow-motion experiment with the young scarlet shiners is still on track. There are currently 7 fish that have been exposed to a higher dose of 11-KT, 10 fish that have been exposed to a lower dose of 11-KT and 5 fish that are controls with no exposure to 11-KT. These aren't extremely strong numbers statistically but as at least a pilot project I hope this shows some pattern of influence on brain development.

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