Cyt-b Gene Sequence Of A Fundulus From Chipola River, Florida
I have a growing number of cytochrome-b gene sequences from a group of Fundulus fish in the lower Appalachicola basin of the Florida panhandle. These are starhead topminnows with a distinctly different phenotype from either F. lineolatus to the east, or F. escambiae to the west. Here's one of the complete 1140 base sequences of a fish from the Chipola River. It hasn't gone through any kind of review, but this is based on a really clean PCR and sequencing with standard primers. Here it is, in 5' to 3' order:
ATGGCAAACCTTCGAAAAACCCACCCCCTTTTTAAAATCGCAAACGACGCTCTA
GTAGACCTACCAGCCCCCGTAAACATTTCCGTGTGATGAAACTTTGGATCCTTA
TTAGGATTGTGTTTAATTGCCCAAATTTTGACCGGGTTATTTTTAGCTATACAT
TATACTTCTGATATTTCTACAGCCTTCTCATCCGTCGCACACATCTGCCGGGAT
GTAAATTATGGCTGGTTAATCCGTAATATACATGCCAATGGTGCTTCCTTCTTT
TTTATCTGCATCTACCTTCACATCGGACGTGGTTTATACTACGGCTCCTACCTT
TATAAAGAAACATGAAATGTAGGTGTTATTTTACTGCTCCTAGTTATGATGACT
GCCTTTGTAGGATACGTTCTCCCTTGGGGCCAAATATCTTTTTGAGGTGCCACC
GTTATTACTAACCTATTATCCGCAGTTCCTTACGTAGGAGATGCATTAGTACAA
TGAATCTGAGGGGGCTTCTCAGTAGACAACGCCACACTCACCCGGTTCTTCGCC
TTCCATTTTCTACTACCCTTTGTAGTGGCAGCCGCTACTATAGTCCACTTAATT
TTTCTTCATGAAACCGGTTCAAACAACCCCATTGGCCTAAATTCCGACGCCGAT
AAAATTTCCTTCCACCCATACTTCTCTTACAAAGATCTCCTAGGTTTCGCTCTC
CTTTTAGCAGCCCTAATCTCCCTAGCCTTATTCTCCCCCAATCTCTTAGGGGAC
CCAGAAAATTTTACTCCAGCTAACCCCTTGGTAACTCCCCCTCATATCAAACCA
GAATGATATTTTCTCTTTGCTTACGCTATTCTCCGATCTATTCCTAATAAACTG
GGTGGGGTCCTAGCCCTGCTAGCATCAATTCTTATCCTAATAGTCGTCCCTATT
CTTCACACATCTAAATTACGAAGTCTAACTTTCCGCCCTCTATCCCAATTCTTG
TTTTGACTATTAGTGGCTGACGTCGCAATCCTAACATGAATTGGTGGTATACCA
GTAGAACATCCCTTTGTGGTTATTGGACAAGTTGCCTCCGTCCTATACTTTTCC
ATTTTCCTGTTTCTTTCACCCGCCGCAGCGACATTAGAAAATAAAATTCTCCAA
TAATAA
1 Comments:
More strings of T's than I expected.
But doesn't look like escambaie at all.
:)
Doug D.
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