Saturday, February 27, 2010

Reorganized For Brain Measurements, DNA Sequencing

I met with Joe, Andrew, Taito and Brittany yesterday afternoon to take stock of where we are after the last two weeks. Ernie has offered to help us do Western blots for NMDAR assessment of non-reproductive season fish, so Taito and Andrew will work with him. We may be able to use a simplified blotting process that Ernie has brainstormed which would be good, but still needs testing. We've lost February as a month in Brittany's study of driftnet contents, but we'll just pick up in March with the next new moon on March 15. Brittany has also offered to work on measuring fish brain size while waiting for more driftnet material to ID. We'll go out this Friday to Limestone Creek to collect scarlet shiners for NMDAR analysis, the last piece of the puzzle. And hopefully as of Wednesday we'll have cleaned up PCR product for 9 individuals to take to Operon for sequencing as a first test of Joe's work on the stippled studfish project. As usual I'm hoping for the best, and I think it will actually work.

This is my 400th post here. And I don't even have a snazzy new photo to post!

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